Sathya B

5 Questions 4 Answers 0 Followers

Questions related from Sathya B

How to determine the concentration of an inhibitor?

I want to calculate the IC50 of a inhibitor compound. The inhibitor was obtained through insilico approaches. Now I need to perform the validation study for the inhibitor. To perform the assay I...

06 June 2017 1,192 1 View

Is duplicate removal needed for RNA seq pooled sample?

I am using pooled RNA seq samples sequenced under the Illumina platform. This data has high sequence duplication at nearly 87%. For finding SNPs is it necessary to remove duplicates after the...

01 April 2014 3,202 1 View

Can anyone help with SNP quality score?

I have found SNP on my datasets using SAM tools mpileup, GATK abd Freebayes software. I can see different SNP quality for the same SNP in each tool. I would like to know what should be the...

12 March 2014 1,281 1 View

Does anyone know how to predict SNPs in a particular gene?

I have data for which differential gene expression was found. Now I am interested in finding SNPs only in the genes which are differentially expressed (gene name is known). Could someone suggest...

27 February 2014 6,447 2 View

Can anyone help with high sequence duplication?

I have performed quality checking on my data. I have found that there is high percentage of sequence duplication (>90%). My next step is aligning my reads to reference sequence. Now I should focus...

17 February 2014 9,774 1 View