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I want to calculate the IC50 of a inhibitor compound. The inhibitor was obtained through insilico approaches. Now I need to perform the validation study for the inhibitor. To perform the assay I...
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I am using pooled RNA seq samples sequenced under the Illumina platform. This data has high sequence duplication at nearly 87%. For finding SNPs is it necessary to remove duplicates after the...
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I have found SNP on my datasets using SAM tools mpileup, GATK abd Freebayes software. I can see different SNP quality for the same SNP in each tool. I would like to know what should be the...
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I have data for which differential gene expression was found. Now I am interested in finding SNPs only in the genes which are differentially expressed (gene name is known). Could someone suggest...
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I have performed quality checking on my data. I have found that there is high percentage of sequence duplication (>90%). My next step is aligning my reads to reference sequence. Now I should focus...
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