Can anyone help with high sequence duplication?

17 February 2014 1 10K Report

I have performed quality checking on my data. I have found that there is high percentage of sequence duplication (>90%). My next step is aligning my reads to reference sequence. Now I should focus on reducing duplication or can I proceed on mapping my reads with duplicated sequences?

Sathya B

It is human data. I have used FastQC tool in that it shows like more than 90% duplicates. It is pooled RNA seq data from 3 different patients which i have taken from DDBJ DRA database.

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