Sarah Shapley

9 Questions 3 Answers 0 Followers

Questions related from Sarah Shapley

Streptavidin AP washing buffers purpose?

Hey all, I'm very interested in understanding more about the biochemistry behind all of the wash buffers that go into the streptavidin affinity purification protocol seen here...

01 May 2023 8,950 4 View

Which transfection reagent to use for which purpose?

Hi there! I've used transfection reagents for different experiments over the years, and I'm curious if there's a reasoning to use JetPrime vs PEI vs Lipofectamine? Do any of a particular benefit...

10 November 2021 5,389 3 View

Image processing protocol for zebrafish myelin?

I'm relatively new to microscopy imaging analysis so I'm seeking some help! I have z-stack images (.czi files) from zebrafish using a Zeiss LSM 880 confocal microscope at 40x water immersion...

30 March 2020 3,032 2 View

Dissolving fish embryos?

Hi everyone, I'm wondering how to fix a problem my lab has been having for quite some time. We have tried two different solutions, both of which seem to dissolve all the zebrafish embryos....

18 February 2020 9,542 1 View

Zebrafish mounting with methyl cellulose?

I'm looking for some help regarding mounting for zebrafish embryos/larvae for live confocal imaging. It seems most protocols in the literature use low MP agarose; however, our lab does not have...

28 December 2019 6,717 1 View

Methods to normalize Western Blots using Cytoskeleton G/F-actin Kit?

I've been using the Cytoskeleton G/F- actin in vivo kit (https://www.cytoskeleton.com/bk037) on my lysates, and am a stand-still with data analysis. I'm torn between whole protein normalization...

04 December 2019 3,040 4 View

Recommendations for Tg actin-RFP Zebrafish?

I am looking for a stable transgenic line of zebrafish which will report RFP polymerized actin (without altering cytoskeletal dynamics). Ultimately, I would like to cross these zebrafish with my...

08 May 2019 376 0 View

How to find peak percentages in ImageJ?

I'm new to Western densitometric analysis, but I have watched various videos and read articles on how to conduct the analysis. My biggest problem is obtaining the peak percentage output on ImageJ....

18 March 2019 3,601 3 View

Systematic method to convert Chromosome ID to SNP rs #?

I am using GWAS data (with plenty of missing cells) which inconsistently lists SNPS with the rs ID or chromosome ID. Is there an automated method to convert from Chr ID to the SNP rs #?

27 June 2018 3,888 1 View