Sarah Sh

5 Questions 2 Answers 0 Followers

Questions related from Sarah Sh

Polysome profiling/ sucrose denisty questions?

This question is regarding the sucrose denisty centrifugation that is used during polysomal fractioning (polysome profiling technique). I was wondering, what is the difference between using let's...

22 March 2016 2,865 1 View

How can I dissolve cisplatin in DMF?

I did not get a product sheet with it so i googled other product sheets for cisplatin(Which has the same molecular formula) and they mentioned I can dissolve it with DMF with "gentle heating".I'm...

11 May 2015 7,550 3 View

Do you test the pH of Towan Buffer when it is 10x or after you diluted it to 1x ?

Hello,I've read a lot online that we are supposed to check that the pH of Towan Buffer is ~8.3 however I couldn't find whether that should be the pH of the 1x buffer after addition of methanol or...

26 January 2015 1,823 3 View

Is YOYO-1 dye stable at 42°C?

I'm trying to study survival of cells under heatshock stress and I want to use YOYO-1 to look at the amount of cells dying. I'm finding that YOYO-1 is not picking up dying cells as properly as it...

17 December 2014 8,114 2 View

I have having issues with my Blots. When I use Ponceau Stain, I see bands but when I probe with antibodies, I cannot detect anything. Any suggestions?

I usually transfer my gel at constant 300 mA for an hour and a half, using a 10% Tris-glycine gel. I use 15% Methanol. So after I transferred and blocked my blot, I incubated the blot with two...

04 July 2014 5,735 4 View