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Questions related from Saptha Vijayan
Has any one tried to check the nitric oxide levels in S2 cells after bacterial infection or LPS/ PGN stimulation? I'm not sure if these cells produce nitric oxide, If they do produce then what is...
06 February 2018 5,764 1 View
I have infected my mice with Mycobacterium marinum. The goal is to perform foot pad histology and do H&E and acid fast stain. Has any one performed footpad histology? Is there any standard...
14 July 2017 5,669 5 View
I transfected Hela cells using PEI (1:3) with a GFP positive plasmid. Six hours post transfection the media was replaced with fresh media. After 72 hours the transfected cells were harvested by...
15 February 2017 5,657 3 View
I have infected the mice foot pad with Mycobacterium marinum and my goal is to monitor the footpad swelling using digital caliper and then check the footpad cfu after termination. My question is...
13 December 2016 1,875 14 View
I need to titrate mouse CD107a antibody to be used for my future CD8+ T cell degranulation assays. After checking the literature, it is recommended to stimulate the cells with some antigen and add...
22 August 2016 539 8 View
I am trying to perform ChIP-seq. I am aiming to achieve a chromatin shear ranging 1000-200 bp. Problem: I ran the reverse crosslinked sheered chromatin sample on 1% agarose gel to check for the...
08 June 2016 6,267 27 View
I set up the digestion as per GeCKO protocol: 5ug- lentiCRISPRv2 3ul- FastDigest Esp3I (thermoscientific) 3 ul- FastAP (thermoscientific) 6 ul- 10X Fastdigest green buffer 0.6ul- 100mM DTT...
04 February 2016 6,426 1 View
