Saptha Vijayan

6 Questions 20 Answers 0 Followers

Questions related from Saptha Vijayan

Mice footpad histology protocol?

I have infected my mice with Mycobacterium marinum. The goal is to perform foot pad histology and do H&E and acid fast stain. Has any one performed footpad histology? Is there any standard...

14 July 2017 5,621 5 View

Does transfected Hela cells survive FACs sorting?

I transfected Hela cells using PEI (1:3) with a GFP positive plasmid. Six hours post transfection the media was replaced with fresh media. After 72 hours the transfected cells were harvested by...

15 February 2017 5,622 3 View

How to measure mice footpad swelling/inflammation?

I have infected the mice foot pad with Mycobacterium marinum and my goal is to monitor the footpad swelling using digital caliper and then check the footpad cfu after termination. My question is...

13 December 2016 1,831 14 View

What is the best protocol to check the expression of CD107a (LAMP-1) by Flow cytometry?

I need to titrate mouse CD107a antibody to be used for my future CD8+ T cell degranulation assays. After checking the literature, it is recommended to stimulate the cells with some antigen and add...

22 August 2016 502 8 View

ChIP- reproducibility with sonicated DNA size range on agarose gel?

I am trying to perform ChIP-seq. I am aiming to achieve a chromatin shear ranging 1000-200 bp. Problem: I ran the reverse crosslinked sheered chromatin sample on 1% agarose gel to check for the...

08 June 2016 6,232 27 View

I have a CRISPR-Problem in digesting plentiCRISPRv2 with BsmBI?

I set up the digestion as per GeCKO protocol: 5ug- lentiCRISPRv2 3ul- FastDigest Esp3I (thermoscientific) 3 ul- FastAP (thermoscientific) 6 ul- 10X Fastdigest green buffer 0.6ul- 100mM DTT...

04 February 2016 6,392 1 View