Santi García

5 Questions 1 Answers 0 Followers

Questions related from Santi García

Using CRISPR technique to perform directed mutagenesis in a mammalian genome?

Dear all, Searching in the literature I've seen a lot of researchs that use CRISPR technique to perform directed mutagenesis in plasmid, vectors or in zigotes, but I didn't find anything about...

28 June 2021 5,347 1 View

Do you know a good FGFR4 antibody for PDX IHC?

Dear all, I've tried many FGFR4 antibodies I saw in the literature but some of them are not useful for IHC and some are only useful for patients slides not for PDX. Do you know a FGFR4 antibody...

15 February 2021 1,259 0 View

How I can compare tumoral samples with their normal paired samples in cBioPortal?

Hi all! I would like to compare the mRNA expression of different genes between tumoral samples and their paired normal samples in TCGA cohorts using cBioPortal. I'm just able to find data from...

20 June 2019 2,949 2 View

N-Cadherin expression plasmid with a selection antibody different than Neomycin?

Hi all! I've using for a long time a construct based on EGFP to overexpress N-Cadherin. Here's the issue, althought I'm supposed to isolate EGFP+ cells (with N-Cadherin expression) by Cell...

09 January 2019 4,304 2 View

Trying to search for a suitable positive control of nuclear/cytoplamic protein extraction technique?

Hi! I'm trying to setting up a nuclear/cytoplasmic protein extraction protocol. I'm using anti-Histone H3 (for the nuclear fraction) and anti-alfa tubulin (for the cytoplasmic fraction) to...

22 May 2018 1,623 1 View