Is it possible to extract total RNA from whole tissue lysate prepared in RIPA buffer and stored at -80 degree?

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Prabodh Kumar

I don't think you should do a qPCR. RIPA generally lyses nuclear membrane which contains many ribonucleases. You may get non-reliable results.

Dalit Talmi-Frank

You can run a qubit and tape station to know if there is any RNA left (although I doubt it) and the quality of RNA...

Good luck