Salman Ul Islam

10 Questions 8 Answers 0 Followers

Questions related from Salman Ul Islam

Need suggestion for stable cell line generation?

Hello All! I am trying to generate HCT 116 stable cell line. I studied many protocols and proceeded as follow: 1) I cultured cells in 6 wells plates at density of 5 x 10^5. 2) At 70% confluency,...

16 November 2016 4,705 3 View

From where can I get plasmid encoding Prp4 gene (my gene of interest)? Can someone suggest some company which provides such services?

I am going to do stable transfection of Prp4. For this I need plasmid which encodes Prp4 gene and is antibiotic resistant. While searching my Lab, I found PRPF4B (NM_003913) Human cDNA Clone...

29 May 2015 1,619 1 View

Did someone use "Prostaglandin E2 Express EIA KIT"?

I am going to check the PGE2 secretion by using Prostaglandin E2 Express EIA KIT, cayman chemical. I am using it for the first time, so little hesitated. I have four samples, as below: (1) Control...

24 February 2015 985 2 View

Does anybody know antibodies for single use?

Everybody knows that antibodies are so costly, ranging from 150-300 USD/antibody.These are available in 100-500 ul quantity. I want to test a cell signaling pathway, for which i need 5-10...

06 February 2015 3,466 6 View

How do I over-express a certain protein in mammalian cell lines?

I want to over-express integrin alpha v Beta 3 in cancer cells line. Does anyone have a suitable protocol for this?

12 January 2015 4,131 3 View

Which proteins expression should I check by western blot for confirmation of apoptosis?

I am checking the apoptosis-inducing effect of a drug on a colon cancer cell line. I have to check the apoptotic pathway proteins by western blot. Please suggest which main proteins I should check...

06 November 2014 1,474 13 View

What is the optimum time before a drug starts generating ROS?

I want to check the production of ROS by using DCF-DA as indicator in Colon cancer cells. What is the optimum time after which a drug starts the generation of ROS? 30 min, 1 hr, 6 hr 12 hrs, 24...

22 September 2014 7,525 10 View

Is this cell staining assay protocol right?

Through MTT assay, i observed that a drug is causing cell death. Now to confirm the cell death pathway, i am expanding my study. I want to check the ROS generation using DCFDA as indicator. In MTT...

16 September 2014 846 4 View

79% Cell viability in MTT assay is indicating induction of apoptosis or Necrosis/Cell Toxic effect of a drug. Can anyone explain this?

I am conducting MTT assay of HCT-15 cells using a drug; concentration is 10uM. I am getting 75-80% cell viability. Actually i want to induce apoptosis in these by using this drug, but my professor...

01 September 2014 5,927 16 View

Final concentration of 100% ethanol is 1% (50ul in 5ml) in cell culture dish, having 5ml cell suspension. Does it affect the cell line?

I have cultured HCT-15 (Adherent), colon cancer cells in 35mm cell culture dish, having 5ml of RPMI-1640 media. I have to treat these with 10uM of a drug. The stock concentration of drug (C1) is...

13 August 2014 7,766 33 View