Sahel Safaei

5 Questions 1 Answers 0 Followers

Questions related from Sahel Safaei

Why are my Caco-2 cells showing abnormal morphology after 5 passages in DMEM high glucose with 10% FBS?

After 5 passages, my Caco-2 cells are exhibiting unusual behavior or morphological changes when cultured in DMEM with high glucose and 10% FBS. What could be the possible reasons for this? Cell...

last month 7,630 0 View

What is the best solvent for lyophilized cell-free supernatant of Lactobacillus for GC–MS analysis?

I have lyophilized the cell-free supernatant of Lactobacillus and tried to re-dissolve it in several solvents including ethanol, methanol, and ethyl acetate, but none of them worked properly (no...

18 August 2025 5,098 0 View

How can I prevent precipitation and aggregation in chitosan–TPP nanoparticles during drug loading and alginate encapsulation?

I am preparing chitosan–TPP nanoparticles for drug delivery with ionic gelatination. My current formulation uses 0.05% chitosan in 1% acetic acid (2.5 mg chitosan in 5 mL; 0.5 mg/mL). The TPP...

15 August 2025 2,881 3 View

Suitable HNMR Solvent for Folic Acid–chitosan Conjugates?

Hello everyone, I’m working on recording the ¹H NMR spectrum of a folic acid–chitosan conjugate. When we attempted to dissolve the sample in D₂O, it did not dissolve properly. The operator added a...

29 July 2025 8,423 2 View

Best fluorescent dyes and protocol for live/dead bacterial staining using PI and Hoechst 33342?

Hello everyone, I'm planning to evaluate the antibacterial effect of a test substance on bacterial cells by using fluorescent dyes for live/dead staining. Unfortunately, I don't have access to a...

01 June 2025 5,836 2 View