8 Questions 16 Answers 0 Followers
Questions related from Sagarika Das
Can someone tell me if 18S rRNA can be used as the reference gene for qRT-PCR analysis if cDNA has been constructed using oligo dT primers? I hear different opinions from people who have and...
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I am working on mono- and sesqui-terpenoid enhancement in tea culture. Can someone suggest some pathway inhibitors which can restrict downstream product formation and enhance the required terpenoids?
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Can someone provide me with a protocol to extract terpenoids from leaves? Literature would be highly appreciated. Thank you.
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Can someone suggest me what type of SPME fiber would be best for extraction of volatile (terpenoid) components from suspension and callus cultures obtained from different explants for analysis...
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Alternate method other than SPME for sample preparation
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I tried callus initiation in MS media containing different concentrations of BAP, Kinetin and 2,4-D in combinations. The same combinations help to initiate and maintain calli from other explants...
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For how long can an SPME fiber be stored after using it for the first time?
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