Sagarika Das

6 Questions 14 Answers 0 Followers

Questions related from Sagarika Das

Can 18S rRNA gene be used as the reference gene for qRT-PCR analysis?

Can someone tell me if 18S rRNA can be used as the reference gene for qRT-PCR analysis if cDNA has been constructed using oligo dT primers? I hear different opinions from people who have and...

11 December 2020 2,260 6 View

Can someone suggest some pathway inhibitors for terpenoid pathway in plants?

I am working on mono- and sesqui-terpenoid enhancement in tea culture. Can someone suggest some pathway inhibitors which can restrict downstream product formation and enhance the required terpenoids?

06 August 2019 4,878 3 View

Why do some callus cultures produce polyphenols?

Why do some callus cultures (out of many) produce polyphenols and how can it be prevented? Is it because of the media composition or some other factors?

05 July 2016 2,973 9 View

Can someone tell me an alternate procedure other than SPME for sample preparation for volatile study from suspension cultures through GCMS?

Alternate method other than SPME for sample preparation

10 May 2016 1,136 4 View

Can someone suggest a better callusing medium for leaves?

I tried callus initiation in MS media containing different concentrations of BAP, Kinetin and 2,4-D in combinations. The same combinations help to initiate and maintain calli from other explants...

24 March 2016 9,906 11 View

Can someone tell me how many times an SPME fiber can be used and for how long can it be stored after using for the first time?

For how long can an SPME fiber be stored after using it for the first time?

18 March 2016 6,264 7 View