Laurence Stuart Dawkins-Hall

Yes, you can but it will be better if you used more than one reference gene

It means that we cannot use 18S rRNa as a reference gene if cDNA has been constructed using oligo dT primer. We can obtain cDNA from 18S rRNA if we have a random hexamer primer.

Sagarika, I've found this informative article:

https://link.springer.com/article/10.1007/s11105-012-0474-y

Thank you everyone for you inputs.

Thank you Anastasia for tye article. It was really helpful.