Sabyata Pharmacy

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Questions related from Sabyata Pharmacy

How do you do Accuracy for Dissolution validation of XR tablets?

Normally Using placebo spiked with drug powder as samples only works for immediate release forms since they disinteregate anyways. But what is to be done for XR tablets ? Would spiking method...

17 May 2018 8,278 6 View

What is the range for inhibitor or non-inhibitor during inhibition assay?

IC50 are meaningless until substrate conc is known right?. Assume competitive binding and the substrate conc is 100uM. If my test compounds are assayed at 100uM and at 10uM, then what %...

02 March 2016 1,921 7 View

Can the same micro plate reader used for ELISA testing be used for enzyme inhibition assay that reads colorimetric absorbance?

I am confused- there is both colorimetric and flurorimetric ELISA which is done in micro plate reader. I am screening enzyme inhibitors and the product says colorimetric assay but it is not a...

26 February 2016 9,856 3 View

Help with protein inhibition assay setup?

i want to DPP4 protein inhibition assay of at least 10 compound with 96 well plates. Both the standard drug (sitagliptin) and the substrate is present at 100uM conc during assay. I want triplicate...

25 February 2016 8,670 6 View

Enzyme inhibition of neutral substance vs its mono or di salt form?

I need to test some docking results by enzyme inhibition assay. But docked compound is neutral while only salt form is found. Should i be concerned?

23 February 2016 8,519 7 View

What is the authoritative way of confirming that a compound's biological in-vitro activity has not been tested before?

in-vitro bioassay are expensive. During Drug discovery, after virtual screening, compounds from Zinc database, how can be confidently assay our compounds knowing that it's concerned activity has...

24 September 2015 6,998 3 View