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Questions related from Ryan De Sotto
I'm new to the field of metatranscriptomics and currently using a software for differential expression of a control vs treatment experiment. I need to use an annotated genome of E. coli Nissle...
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We have a standard curve for the analysis of compounds in water. Method is done using Triple Quadrupole LC-tandem MS. How do we compute for LOD and LOQ? We didn't measure blanks (that's the common...
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I just need a clear explanation why some metabolites are upregulated or downregulated in the presence of a xenobiotic compound? I do want to know the general mechanism though. Like example, a...
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I am doing a degradation experiment using a white rot fungi grown in PDB with yeast extract for 7 days. After which, it's washed with phosphate buffer 4.5. I just want to check the capability of...
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We use terbutylazine as our internal standard for analysis of pharmaceuticals in water. Should we put it before or after SPE?
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I have some pellets of P chrysosporium grown in PDB and YE for 7 days at 30 degrees. These pellets were washed with phosphate buffer and supplied with an energy source (glucose) for a degradation...
30 July 2014 9,746 1 View
I have some samples for metabolite analysis using Q-TOF and I need to evaporate the solvents using rotary vapor. I am just not sure which settings would be the best choice (rpm,temperature and...
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