Ryan De Sotto

5 Questions 8 Answers 0 Followers

Questions related from Ryan De Sotto

Where can I download a fully annotated genome of an organism for transcriptomics analysis?

I'm new to the field of metatranscriptomics and currently using a software for differential expression of a control vs treatment experiment. I need to use an annotated genome of E. coli Nissle...

05 January 2021 9,329 3 View

Why are metabolites upregulated or downregulated in the presence of a xenobiotic compound?

I just need a clear explanation why some metabolites are upregulated or downregulated in the presence of a xenobiotic compound? I do want to know the general mechanism though. Like example, a...

13 January 2015 7,472 13 View

Does anyone have knowledge on the Glucose Concentration in Degradation Experiments Involving White Rot Fungi?

I am doing a degradation experiment using a white rot fungi grown in PDB with yeast extract for 7 days. After which, it's washed with phosphate buffer 4.5. I just want to check the capability of...

09 September 2014 4,284 5 View

Should internal standards be added before or after SPE?

We use terbutylazine as our internal standard for analysis of pharmaceuticals in water. Should we put it before or after SPE?

10 August 2014 5,036 15 View

How much time do we need to evaporate organic solvents in a 2-mL solution (frozen under -80 degrees) using a rotary vacuum (Scanvac)?

I have some samples for metabolite analysis using Q-TOF and I need to evaporate the solvents using rotary vapor. I am just not sure which settings would be the best choice (rpm,temperature and...

27 July 2014 1,917 10 View