Rut Christine Inggriani

10 Questions 15 Answers 0 Followers

Questions related from Rut Christine Inggriani

My Infusion cloning with takara not working?

Hello, I tried to clone 3 insert into plasmid pUC18. I used Takara infusion cloning with this reaction: 1 μl 5X In-Fusion HD Enzyme Premix 0.59 μl Linearized vector (2667bp, DNA mass=44.35...

23 January 2024 3,434 3 View

Is there any web based tools using rsID to know SNPs - SNPs interaction?

Hello, I have the SNPs ID list from the VEP Ensembl database based on the whole exome sequencing data I got in the literature. I want to know if there are any web-based tools that can analyze the...

19 September 2022 5,681 1 View

How to find TCGA about ovarian clear cell carcinoma (CCC) and endometrioid carcinoma in GDC?

I need data TCGA about endometriosis-associated ovarian carcinoma (EOAC) which consists of clear carcinoma and endometrioid carcinoma. I have been trying to use GDC data to filter TCGA data but...

12 February 2022 9,562 0 View

Why do I lose my PCR product when decreasing the volume reaction?

I used volume reaction 20µl volume with 5µl for template RNA. But recently I used 10µl volume reaction with 2.5µl template RNA. I used one step TaqPath Assay IC-VDE from thermo fisher with the...

15 May 2021 2,010 9 View

Why there are two peak and low intensity fluorescents in my positive control?

I am performing qRT-PCR for covid 19 using IC-VDE kit but there are two peak showed up in my positive control. The intensity also very low, below 1. Sometimes I found the sample also have ct...

08 April 2021 3,809 3 View

How to calculate r^2 for LD using r analysis?

I have two SNPs from FTO gene and i want to analysis their LD with R analysis. How to calculate r^2 for these two SNPs? I have already calculate the D' with script LD (rs1421085, rs9939609) and i...

15 December 2017 6,844 3 View

Does anyone know about SNP FTO in human exon region?

I'm doing my research about FTO gene in human. I want to find any SNPs in human exon region. Does anybody know about this? Is there any research about this? Are SNPs in FTO gene only associate...

04 September 2017 297 6 View

Whole exome sequencing database

I am doing a research about endometriosis-associated ovarian cancer and focus on whole exome sequencing data. While I am doing literature review to find the clinical data and whole exome database,...

01 January 1970 7,629 9 View

How to get gene expression data from list of gene?

Hello, I have the gene of the list of whole exome sequencing data from the paper. Can I use this list of genes to get the gene expression data? Should I download the transcriptomic data using this...

01 January 1970 8,625 3 View

How to do variant filtration after annotation in exome sequencing?

I have collected exome data from a few literature articles. I got a thousand variants and would like to proceed in the next step which is variant filtration to determine the gene of interest. How...

01 January 1970 8,739 2 View