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Hi, I am doing in vitro reaction with recombinant substrate protein and kinase. We got the activity of the kinase, 16nmol/min/mg, how much substrate protein and kinase should I use? And how much...
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I am doing in vitro reaction with recombinant substrate protein and kinase. We got the activity of the kinase, 16nmol/min/mg, how much substrate protein and kinase should I use? And how much...
10 December 2018 7,585 0 View
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Hi, I am doing in vitro reaction with a kinase and its potential substrate protein. We got recombinant kinase and substrate protein. And we know activity of the recombinant kinase is 16...
10 December 2018 5,442 0 View
I am preparing samples for phosphoproteomics. After reduction, alkylating, and digestion, I acidified my cell lysates with 20% TFA before purifying the digest with Sep-pak. When I digested ~1.5mg...
12 January 2018 6,771 4 View
I am trying to do a targeted phosphoproteomics project. We don't have LC-MSMS in our lab, so we have to send out our prepared samples to collaborator. Before I send them out, is there any way that...
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