Pranali Patil

6 Questions 6 Answers 0 Followers

Questions related from Pranali Patil

How to test the efficacy of TTX in blocking sodium channel currents? AND What are the typical waveforms of mEPSC's and mIPSCs?

I am recording mEPSC (at -80mV, 2micromolar TTX) and mIPSC (at -60mV, 2uM TTX, 20uM APV, 50uM DNQX) in amygdala C57 mice (8 to 10 weeks). there is a report which says a sodium resistant channel...

26 March 2021 7,848 9 View

Logic of internal solution in patch clamp electrophysiology?

What factors should one consider while selecting concentrations of the internal solutions in the micropipette used in patch clamp? What Ion concentrations are optimum for mEPSC and mIPSC? Please...

19 January 2021 6,153 3 View

Can you use open drop / Bell Jar method of anaesthesia for ACSF perfusion?

Can you use the open drop / Bell jar method, to administer isoflurane on a cotton gauze and once the mice don't show righting reflex or reaction to toe pinching, plus the breathing has slowed...

14 January 2021 214 0 View

Cfos or Arg3.1, which is better? References for Comparison between different IEG's?

I am planning to do experiments where I want to tag neurons involved in memory engram after auditory fear conditioning in mice and then look at the temporal profile of which neurons get tagged...

12 January 2021 5,690 0 View

Does Lucifer Yellow interfere with miniEPSC/IPSC recordings?

I want to mark the neurons I am recording from during whole cell Patch clamp. I am thinking of using Lucifer Yellow. Does it increase noise during recording? What are the better alternatives to...

05 January 2021 8,103 3 View

Overexpressed proteins appear vesicular vs. the endogenoue construct which appear soluble. What could be the reason for this?

I am looking a axonal transport in drosophila larval sensory neurons. When i compared the endogenous expression vs. the overexpression constructs of the following proteins i saw soluble vs...

22 December 2018 6,178 0 View