Prakasam Gopinath

4 Questions 8 Answers 0 Followers

Questions related from Prakasam Gopinath

Is the Warburg effect phenomenon, shown in cancer cells really a critical therapeutic target ?

Cancer cells were shown to exclusively prefer Aerobic Glycolysis over Mitochondrial oxidation phosphorylation. Well literature has shown several advantages of cancer cell by performing Warburg...

01 January 2015 9,141 10 View

Can anyone provide me Lentiviral transfer vector (2nd generation) for the expression of desired gene of interest?

I want to over express my gene of interest in HeLa cell line, I will be highly grateful if some one can provide me Lentiviral transfer vectors (2nd generation with GFP/RFP). I do have psPAX2 and...

12 December 2014 7,914 4 View

Can any one suggest me a best protocol to extract metabolites from Human cancer cell lines?

I wish to quantify the metabolic intermediates (Glycolytic and TCA intermediates) between the cancer cells, treated with different small molecule activator and inhibitors of specific...

12 December 2014 4,988 5 View

How to quantify the cytoplasmic and nuclear concentration of metabolites (ATP)?

I want to know concentration of Adenine Nucleotides concentration of nucleus

11 November 2013 7,972 0 View