Piers Gillett

6 Questions 8 Answers 0 Followers

Questions related from Piers Gillett

Do empty wells in the BioRad CFX 384 require water?

When running a 384 well plate on the CFX 384, I won't necessarily be filling every single well with some kind of sample or control. Do I need to fill the empty wells with water or something or can...

06 June 2017 737 3 View

Would results from experiments involving co-culturing of SY5Y (human neuroblastoma) and BV2 (mouse microglia) cells have validity?

A few papers (attached) have co-cultured human brain cells with mice BV2 cells. I am wondering as to the validity of any study employing such tactics. My initial thoughts when hearing of this is...

01 January 2015 5,088 2 View

How fast do BV2 cells divide?

This is the first time I will be culturing BV2 cells although I have worked with SH-SY5Y previously. I am looking to find out how long BV2 take to divide and therefore when they need to be seeded...

12 December 2014 277 6 View

Is there a program available that shows the degrees of separation between proteins that do not interact?

I am looking for a tool, preferably online and freely available but it will tell you the proteins that would link 2 proteins that do not interact themselves. For example, the protein products of...

12 December 2014 408 5 View

What is the difference on gene silencing effects between 5-hydroxymethylcytosine and 5-methylcytosine?

I am interested in terms of their effects on gene silencing. I had not heard of hydroxymethylcytosine until today and would like to find out how this modifications differs in terms of silencing to...

12 December 2014 7,098 2 View

Why are Cryopreserved mouse brain sections are drying out?

I am undertaking immunohistochemistry on sections from a knockout mouse line we have. The issue I am having is that in the final steps of the protocol, a number of ethanol baths (50%, 70%, 95%,...

09 September 2014 693 3 View