Pengfei Ding

4 Questions 16 Answers 0 Followers

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How to prepare positively charged EM grids?

I do not have any glow discharger available. What reagents can I use to get the grids positively charged? Or are there positively charged EM grids commercially available?

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How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

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Which spectrometer should I use to monitor Protein-DNA interaction?

I want to use fluorescent anisotropy(polarization) to detect protein-DNA binding. The signal of tryptophan is different with or without DNA binding. I know that PTI QuantaMaster spectrometer can...

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Can I add high concentraion NaCl into my GST buffer?

I am using GST-affinity tag to purify my protein. The standard protocol inculdes ~150mM NaCl in the GST buffer. In order to remove the DNA (maybe interact with my protein), I want to increase the...

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