Pardha Saradhi Attuluri

6 Questions 22 Answers 0 Followers

Questions related from Pardha Saradhi Attuluri

Filing solution for organic nonaqueous pH probe ?

Hello, I want to measure/adjust the pH of tetrahydrofuran (THF) of different dilutions with water. After reading brochures some of the pH meters, I get to know that LiCl in Ethanol should be used...

05 November 2019 9,113 0 View

Calculate Time frame for UV treatment for cells?

Hello I would like to conduct a mock experiment on plant cells nucleotide excision repair. the UV sourse I have is a tube light used in laminar hood. UV lamp specifications: emmission @254nm...

18 January 2018 403 7 View

Why do I detect no insert after cloning into pENTR D TOPO?

lenght of my inserts vary from 450 to 1.5 kb. all amplification primers have CACC at 5' of forward primer. after topo reaction(followed as instructed in manual) got colonies. when i did colony PCR...

29 July 2015 993 7 View

How can I make 2x or 3x fluorescent gene constructs?

I would like to make 3x tagRFP for increasing brightness of target protein. Can you please suggest different methods? Thanks:).

19 May 2015 492 2 View

Can we stain mitochondrial DNA with EdU/BrdU?

Can the mitochondrial DNA replication be labelled with EdU or BrdU?

20 March 2015 5,264 5 View

Why does fluoresce of FPs decreases in organic solvents?

few articles report that, fluorescence remains constant in ethanol dehydrated tissue upto 30 min (not given data after that time point). Addition of hexane decreased it to more than 50%.

13 March 2015 3,881 2 View