7 Questions 5 Answers 0 Followers
Questions related from Pannapa Pinweha
Dear all, I've try to purify the digested plasmid (16 kb) from the agarose gel using the gel purification kit. Anyway, I got very low concentration of the plasmid after purification. And I cannot...
11 November 2017 2,800 1 View
Hi everyone I did the 25 bp deletion as series in my 3'UTR region for observing which regions are important for miRNA regulation. Now I got the region that may be regulated by miRNAs but I don't...
02 February 2017 5,940 4 View
I would like to transfect the mimic miRNA into the cells. And I have some questions. 1. Is the Lipofectamine 2000 suitable for mimic miRNA transfection? 2. Does anybody has the experience in...
12 December 2015 9,005 8 View
Hi everyone, I would like to know that if my normalized gene " 18srRNA" was detected at ct=9 but my interested gene was detected at ct=29, I would like to know that why is the low ct not good for...
10 October 2015 7,083 3 View
I would like to check the cDNA after the stem loop RT-PCR reaction done. In the normal RT-PCR, the cDNA can check by B-actin amplification. But stem loop RT-PCR is different. So can anyone tell...
10 October 2015 5,892 5 View
I would like to measure miRNA expression by stem-loop RT PCR. I case of normalizer, I would like to use U6 snRNA by using SYBR green system. That's it possible.
10 October 2015 4,630 8 View
Does anybody have a substrate equilibrium buffer recipe for EMSA? I have tried to search this recipe for a long time if anybody known it. Please inform me. Thank you so much.
05 May 2015 4,152 1 View
