Hi! I recommend using LatchBio for RNA seq data. I've used it several times, and it is super easy to use since it has a non-coding interface.

Hope that helps!

https://latch.bio/

#Jasmine Gallegose

Thabk you for the suggestion. Can you explain in brief stepwise , how ot works. That will be really a great help.

Thanks in advance.

previously SOTA now everyone performs Deseq2.

if you perfect genome map you can carry on map-based alignment coupled Deseq2. If you dont have good quality genome peform de novo assembly with SPADes an than later on carry on any feature selection tools you would like.

Check out this article for performing PCA on on RNA-seq expression data https://www.reneshbedre.com/blog/principal-component-analysis.html