Mohammed Ismael

10 Questions 9 Answers 0 Followers

Questions related from Mohammed Ismael

How to produce neurospheres from parental glioma cell lines?

Hello, We are currently trying to produce neuerosphere cultures from parental glioma cells lines. Parental cells were disassociated and cultured in neurobasalA serum free media containing...

07 March 2019 9,014 2 View

What are the most important publications in biology/molecular biology in the last 100 years?

Hi, If you could name the most important publications relating to any of the biological sciences over the last 100 years what would those be? Beginning with Francis & Crick's paper on...

25 July 2018 7,945 4 View

What is the difference between 47s and 45s rRNA in humans?

In humans, the rDNA is found along the nucleolar organiser regions, transcription of rDNA begins through a pre-ribosomal rRNA transcript, in some places it is referred to as 47s in others 45s,...

30 June 2018 4,202 2 View

How to make up MilliMolar or Molar solution from lyophilized powder?

Hello Friends, I have a drug, 125ug in lyophilized form. mW is 1255.42 If I add 250ul of D.H20 to this I get 0.5mg/ml solution. I need to have a final working concentration of between 1-5ug/ml to...

30 May 2016 1,904 2 View

How do you convert an X-ray dose into a Proton dose?

Hi, I am irradiating some mammalian cells comparing both X-rays and protons. I know what dose I am exposing my cells to using X-rays, for example 4Gy, but if I want to radiate with protons how can...

19 February 2016 6,535 7 View

Do any DNA or RNA repair proteins localise to the nucleolus upon exposure to radiation?

Hello, Does anyone know of any DNA/RNA repair proteins that can be used as a marker to detect localised damage within the nucleoli of cells - such as exposure to radiation? Nucleoli are made up of...

14 January 2016 6,340 9 View

Which melanoma cell line has good nucleolar morphology?

Hello, Anyone know of any good melanoma cell lines which display clear, distinguishable (and preferably large) nucleoli and also with known p53 status please? Thank you

16 October 2015 3,301 3 View

Is there a Glioblastoma cell line with p53 positive status?

Hello, I am currently working with LN18 which has a p53 mutated status. I would also like to experiment on another glioblastoma cell line which has a p53 non mutated status, can anyone recommend a...

16 October 2015 3,188 4 View

How can I choose fluorescent antibody for cell imaging?

Hello, I am attempting to image the three compartments of the nucleolus. I have chosen RNA Pol1 as a marker for the fibrillar centre, fibrillarin as a marker for the dense fibrillar centre and B23...

16 October 2015 6,321 5 View

How can I dissolve a drug in DMF?

Hello, I purchased a drug and the data sheet said to dissolve in DMF. I purchased 5mg, calculations showed I needed to add 486ul to my 5mg powder to make a 20mM stock. When I added the DMF the...

07 October 2015 1,663 5 View