Mohammad Pirouzfar

8 Questions 14 Answers 0 Followers

Questions related from Mohammad Pirouzfar

How to analyze spectrophotometry data of mitochondrial enzymes?

Hi everyone, I am measuring the activity of some mitochondrial enzymes, by measuring the oxidation/reduction of relevant substrate using spectrophotometer. However, after analysis, my enzymatic...

04 April 2023 6,198 1 View

Is there any established method for in vitro transcription of genes with CMV pomoter?

Hi all, I am planning to have the in vitro transcript of my gene of interest which is under the control of CMV-IE promoter. Is there any commercial kits or any established method for that?

11 January 2023 9,295 1 View

Anyone knows about bioinformatics tool for checking interaction between viral miRNA and human mRNA?

I am looking for a bioinformatics tool preferably online which can help me detect binding of my viral miRNA with 3' UTR region of human mRNA. Some of the tools that I found online are species...

05 January 2023 3,020 4 View

What is a suitable polymerase enzyme for tetra-primer ARMS PCR method?

Hello I am looking for some appropriate Taq pols for my ARMS PCR reaction. Should those enzymes have the 3' to 5' exo activity? Thanks in advance

25 April 2021 2,750 1 View

In a thermocycler machine, is it normal to have a temperature inconsistency between what is displayed and what is measured?

Hi, I've been curious about the performance of a PCR machine, so I measured the temperature of its block with its lid open as it was running in 95 C for a couple of minutes. However, the...

17 March 2021 8,772 2 View

What would be an efficient solution for labeling cryovials and racks in nitrogen tank?

While everyday sticky labels and tapes tend to detach after a short while, and permanent markers are useless in the most of cases, is there any kind of physical labels specially made for labeling...

01 February 2021 1,979 5 View

Alternative qPCR data analyser to ABI 7500 software?

Hi, I have problems with installing ABI 7500 Software on Win 8.1. Is there any alternative software to open .eds files on my OS?

26 December 2018 3,453 1 View

SOLVED: Any one has the experience of transfecting HeLa cells with px-458 plasmid by Calcium phosphate method?

Hi I'm trying to transfect my HeLa cells with addgene pSpCas9(BB)-2A-GFP (PX458) plasmid by CaP transfection method. However, after a long time of trial and errors, my transfection efficiency is...

12 August 2018 1,483 2 View