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Hi everyone, I am measuring the activity of some mitochondrial enzymes, by measuring the oxidation/reduction of relevant substrate using spectrophotometer. However, after analysis, my enzymatic...
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Hi all, I am planning to have the in vitro transcript of my gene of interest which is under the control of CMV-IE promoter. Is there any commercial kits or any established method for that?
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I am looking for a bioinformatics tool preferably online which can help me detect binding of my viral miRNA with 3' UTR region of human mRNA. Some of the tools that I found online are species...
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Hello I am looking for some appropriate Taq pols for my ARMS PCR reaction. Should those enzymes have the 3' to 5' exo activity? Thanks in advance
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Hi, I've been curious about the performance of a PCR machine, so I measured the temperature of its block with its lid open as it was running in 95 C for a couple of minutes. However, the...
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While everyday sticky labels and tapes tend to detach after a short while, and permanent markers are useless in the most of cases, is there any kind of physical labels specially made for labeling...
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Hi, I have problems with installing ABI 7500 Software on Win 8.1. Is there any alternative software to open .eds files on my OS?
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Hi I'm trying to transfect my HeLa cells with addgene pSpCas9(BB)-2A-GFP (PX458) plasmid by CaP transfection method. However, after a long time of trial and errors, my transfection efficiency is...
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