Mohammad Pirouzfar

10 Questions 17 Answers 0 Followers

Questions related from Mohammad Pirouzfar

Does ROS increase oxygen consumption rate (OCR) in the Seahorse Mito Stress Test Assay? If so, in which phase?

Since reactive oxygen species (ROS) can influence mitochondrial respiration, I am wondering whether elevated ROS levels contribute to an increase in OCR during the assay; and if so, at which phase...

18 February 2025 9,664 2 View

Is it appropriate to normalize enzyme activity based on its expression levels?

I am measuring the specific activity of an enzyme under two different conditions using an enzymatic kinetic assay. In one of my conditions, I know that the expression of the enzyme is highly...

05 September 2024 3,435 7 View

How to analyze spectrophotometry data of mitochondrial enzymes?

Hi everyone, I am measuring the activity of some mitochondrial enzymes, by measuring the oxidation/reduction of relevant substrate using spectrophotometer. However, after analysis, my enzymatic...

04 April 2023 6,245 1 View

Is there any established method for in vitro transcription of genes with CMV pomoter?

Hi all, I am planning to have the in vitro transcript of my gene of interest which is under the control of CMV-IE promoter. Is there any commercial kits or any established method for that?

11 January 2023 9,349 1 View

Anyone knows about bioinformatics tool for checking interaction between viral miRNA and human mRNA?

I am looking for a bioinformatics tool preferably online which can help me detect binding of my viral miRNA with 3' UTR region of human mRNA. Some of the tools that I found online are species...

05 January 2023 3,068 4 View

What is a suitable polymerase enzyme for tetra-primer ARMS PCR method?

Hello I am looking for some appropriate Taq pols for my ARMS PCR reaction. Should those enzymes have the 3' to 5' exo activity? Thanks in advance

25 April 2021 2,793 1 View

In a thermocycler machine, is it normal to have a temperature inconsistency between what is displayed and what is measured?

Hi, I've been curious about the performance of a PCR machine, so I measured the temperature of its block with its lid open as it was running in 95 C for a couple of minutes. However, the...

17 March 2021 8,824 2 View

What would be an efficient solution for labeling cryovials and racks in nitrogen tank?

While everyday sticky labels and tapes tend to detach after a short while, and permanent markers are useless in the most of cases, is there any kind of physical labels specially made for labeling...

01 February 2021 2,025 5 View

Alternative qPCR data analyser to ABI 7500 software?

Hi, I have problems with installing ABI 7500 Software on Win 8.1. Is there any alternative software to open .eds files on my OS?

26 December 2018 3,508 1 View

SOLVED: Any one has the experience of transfecting HeLa cells with px-458 plasmid by Calcium phosphate method?

Hi I'm trying to transfect my HeLa cells with addgene pSpCas9(BB)-2A-GFP (PX458) plasmid by CaP transfection method. However, after a long time of trial and errors, my transfection efficiency is...

12 August 2018 1,525 2 View