7 Questions 11 Answers 0 Followers
Questions related from Mohammad Ali Obeid
Can anyone suggest the best medium to grow the Human nasal epithelial cells? I have RPMI, DMEM but I am not sure if they are suitable? I got these cells from Sigma. thanks
03 January 2021 6,172 2 View
Hi, I am doing FACS experements to assess the GFP inhibition by siRNA. I am using A549 cells that are producing GFP and treated the cells with siRNA to inibit the GFP production. the cells then...
03 January 2017 4,045 3 View
If I wrapped the 12 well plates after seeding the cells with alaminium foil and put it in the incubator, would that affect the growth of the cells? or would that affect the O2 levels that reach to...
03 August 2016 10,002 4 View
On what bases can i chose the hydration media for liposomes/niosomes production? some are using PBS or HEPES or just DW, so what are the effects of the media on the resulted particles? thanks
21 January 2016 5,692 4 View
Does anyone work with the Ribogreen assay to measure the siRNA concentration? I have tried it several times but then the standard curve although it's linear but it's very different from the one on...
20 October 2015 8,832 1 View
Does anyone know how I can measure the encapsulation inefficiency of siRNA inside liposomes? Any other method other than the ribogreen assay?
09 June 2015 2,096 12 View
siRNA delivery in Bilosomes
03 March 2015 274 2 View