Mohammad Ali Obeid

10 Questions 20 Answers 0 Followers

Questions related from Mohammad Ali Obeid

Human nasal epithelial cells growth medium?

Can anyone suggest the best medium to grow the Human nasal epithelial cells? I have RPMI, DMEM but I am not sure if they are suitable? I got these cells from Sigma. thanks

03 January 2021 6,205 2 View

How to measure GFP expression with FACS?

Hi, I am doing FACS experements to assess the GFP inhibition by siRNA. I am using A549 cells that are producing GFP and treated the cells with siRNA to inibit the GFP production. the cells then...

03 January 2017 4,083 3 View

Effect of wrapping the cells with foil?

If I wrapped the 12 well plates after seeding the cells with alaminium foil and put it in the incubator, would that affect the growth of the cells? or would that affect the O2 levels that reach to...

03 August 2016 10,107 4 View

How can I measure the niosomes concentration after preperation?

Hello, Can anyone advice how can i measure the concentration of niosomes after preperation? I tried HPLC but can't get seperated peakes. Do i need to lyse the particles before running them on...

29 March 2016 7,528 9 View

Niosomes stability

Hi, I have prepared my niosomes using HEPES buffer and test their stability at 4,25,37 and 50C. I have found they are stable now for about one month at all the tempretures except the 4C where the...

18 February 2016 6,319 2 View

What are the differences in the hydration media for liposomes/niosomes production?

On what bases can i chose the hydration media for liposomes/niosomes production? some are using PBS or HEPES or just DW, so what are the effects of the media on the resulted particles? thanks

21 January 2016 5,722 4 View

How can i increase the stability of the cationic niosomes?

I have prepared cationic niosomes composed of surfactants and DC-Cholesterol but i found these niosomes were not stable and tend to agregates in a couple of days although the ZP was around 75mV,...

20 January 2016 7,951 4 View

Does anyone work with the Ribogreen assay to measure the siRNA concentration?

Does anyone work with the Ribogreen assay to measure the siRNA concentration? I have tried it several times but then the standard curve although it's linear but it's very different from the one on...

20 October 2015 8,865 1 View

How i can measure the encapsulation efficiency of siRNA?

Does anyone know how I can measure the encapsulation inefficiency of siRNA inside liposomes? Any other method other than the ribogreen assay?

09 June 2015 2,131 12 View

Has anyone tried the encapsulation of siRNA inside bilosomes?

siRNA delivery in Bilosomes

03 March 2015 317 2 View