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Questions related from Meme Ali
Hello, I'm really struggling with the coating. I coated my plate using PDL with a concentration of 1 mg/ml. To make a working concentration of 10 μg/ml, I took 1 ml from the stock and diluted it...
03 June 2023 4,009 0 View
Hello I made a glycerol stock for my E. coli, but after 3 months of storage at -80°C, I took some to regrow and got zero bacterial growth. I'm not sure why this happened. We used pure 100%...
23 April 2023 5,399 2 View
Hi I want to measure action potential in response to GFP intensity and I do not have a high-speed camera any recommendations for using fluorescent microscopy? so I am planning to trigger action...
19 April 2023 4,013 3 View
Hello I need a software help me to process my time lapse study thank you
13 January 2023 7,363 1 View
I need to measure the GPF intensity in response to the pH change the issue I am facing is that the GFP is attached inside the cell and I do not know how to measure the intracellular pH with GFP...
03 January 2023 6,867 4 View
hi the question I have done GFP plasmid transfection to the neuron cell this GFP plasmid is pH sensitive I need a way to make sure that the GFP plasmid is working by detecting membrane potential....
27 December 2022 393 4 View
I have been asked to make 100 micromolar and added to the cell culture I used this calculation : 1. Moles = Molarity (M) x Volume (L) I decided to make 0.5 M as a stock solution this dilute it...
23 December 2022 4,664 4 View
Hi i am doing so reserch on measuring neuron activity( action and rest potential) . I need your help to find best neuron cell line that has NADPH receptor and can form axon. I worked with HT-22...
29 November 2022 6,862 2 View
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01 January 1970 5,837 1 View
as you can see from the attached picture 1) how I can avoid cell aggregation. I have tried everything and still, it's frustrating because it affects my picture quality. What kind of neuron is N2a?...
01 January 1970 1,919 7 View
Dear please help I have cell culture ( neuron cell line), I need to measure the cell potential ( rest and action). In our lab I only have to use fluorescent microscope. I have two dyes 1) is...
01 January 1970 9,979 0 View
