I faced with a problem in Qiime2 when I was demultiplexing my data. The error is below:
There was an issue with loading the file Barcodemetadate.txt as metadata:
Metadata file path doesn’t exist, or the path points to something other than a file. Please check that the path exists, has read permissions, and points to a regular file (not a directory): Barcodemetadate.txt
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2019.1/tutorials/metadata/
I have already checked my metadata file with Keemei. It sent me ‘Good Job’! i did not have any error on Keemei!
My data have barcodes which they must be demultiplexed then denoised. I used the only option in the tutorial- qiime demux emp-paired.
My data is Casava 1.8, already imported to .qza artifact. The next step is multiplexing.
Metadata is ready and already cheeked by Keemei. It reported no errors.
The command used I is:
https://docs.qiime2.org/2019.1/tutorials/atacama-soils/
qiime demux emp-paired \
--m-barcodes-file MyMetadataFileName.txt \
--m-barcodes-column Columename \
--i-seqs Artifcatname.qza \
--o-per-sample-sequences demux.qza \
--p-rev-comp-mapping-barcodes
Who can send me a relevant command to demultiplex my data? Or Somebody has an any Idea?
Thanks
why don't you try to use .tsv format for your metadata and use that file in the command you are using.
Abhijeet Singh Dear Abhijeet Singh thank you for the reply. As a matter of fact, it said that Qiime2 can recognize some formats, such as .tsv, .txt, .qza and so forth. Because of that I did not think so it should be a matter. I will try it.
Dear Abhijeet Singh
I run the command by .tsv format but I got the same error!
Do you have any idea?
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