Md. Hafiz Uddin

6 Questions 11 Answers 0 Followers

Questions related from Md. Hafiz Uddin

How I can reduce the cost of new antibody production?

I need new antibodies for different phospho sites for a human protein of my interest. I am contacting different companies. Polyclonal antibody production in rabbit host is expensive. Is it OK if I...

07 January 2020 817 4 View

What are the best markers (genes) to determine different DNA damage responses?

After drug treatments I would like to check a number of genes by qPCR to determine involvement of different DNA damage pathways namely homologous recombination (HR), NHEJ, MMEJ etc. Which genes...

23 March 2018 6,271 4 View

Is there well established cell line for primary peritoneal carcinoma (PPC)?

PPC is a rare type of cancer having extensive similarity with ovarian epithelial cancer. I am dealing with primary cell line of PPC and looking for well established one for comparison. Is it a...

13 February 2015 6,872 2 View

How to evaluate/validate primary cancer cell line?

I have isolated primary cancer cells from ascites. I would like to know the proper way to evaluate/validate primary cancer cell line before any kind of study. I just know that I need to confirm...

21 February 2014 8,234 1 View

Does IC50 value reduce as the passage number of cell increases?

In SKOV-3 ovarian cancer cell line I determind the IC50 value for certain drug at passage number 6. However, at 36th passage I am getting IC50 value about half of previous one. Is that usual? The...

25 November 2013 5,493 9 View

Do I need to filter after dissolving drugs in DMSO?

I am preparing drug stock in DMSO. To take the weight of drug I need to expose it outside the cabinet. Thus I am wondering about microbial contamination. Do I need to filter it with 0.22um syringe...

25 July 2013 904 18 View