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Questions related from Maria Rompf
We need to analyze budding yeast cell extracts by Western blotting, and are looking for a good antibody against a "housekeeping" protein, serving as loading control (actin, tubulin, Gdh, etc.)....
26 March 2014 8,756 11 View
I am studying the effects of starvation in yeast mutants, and looking for some good comprehensive resources about different types of starvation. For example, which cellular response would be...
20 November 2013 160 0 View
I am looking for numbers of amino acid abundance in the total proteins of different species, e.g. E.coli 3% Ala, 5 % Gly...; S.cerevisiae 5% Lys, 2.5% Pro... Are there online databases or papers...
30 July 2013 1,061 1 View
I have a number of yeast mutants in a particular standard S.cerevisiae background, and for some inexplicable reason, the growth behaviour of the wildtype is very strange recently. I normally...
03 July 2013 1,066 15 View
I would like to investigate the role of certain amino acids for survival of S. cerevisiae cells. To this end, I would like to deplete the cells for an amino acid of interest by incubating in media...
07 May 2013 6,934 0 View
I have a microscopy video (frames as stacks in a .tiff) which was acquired using a times series consisting of: 13 frames from time 0 to time 2:00, 1 frame taken every 10 s, 12 frames until 12:00...
12 April 2013 6,385 14 View
I am looking for some numbers of amino acid concentrations in intracellular compartments of mammalian cells (cytosol, endosome, lysosome,...). Extensive Google and Pubmed searches gave me a lot of...
17 March 2013 2,371 3 View
