Maria Rompf

6 Questions 13 Answers 0 Followers

Questions related from Maria Rompf

Can anyone recommend an antibody to use on Western blot as a loading control for S. cerevisiae cell extracts?

We need to analyze budding yeast cell extracts by Western blotting, and are looking for a good antibody against a "housekeeping" protein, serving as loading control (actin, tubulin, Gdh, etc.)....

26 March 2014 8,742 11 View

Resource for abundance of amino acids in different species?

I am looking for numbers of amino acid abundance in the total proteins of different species, e.g. E.coli 3% Ala, 5 % Gly...; S.cerevisiae 5% Lys, 2.5% Pro... Are there online databases or papers...

30 July 2013 1,053 1 View

Inexplicable bad growth of S cerevisiae wildtype - any ideas for troubleshooting?

I have a number of yeast mutants in a particular standard S.cerevisiae background, and for some inexplicable reason, the growth behaviour of the wildtype is very strange recently. I normally...

03 July 2013 1,060 15 View

Yeast starvation for a certain amino acid - how long to deplete the intracellular pool?

I would like to investigate the role of certain amino acids for survival of S. cerevisiae cells. To this end, I would like to deplete the cells for an amino acid of interest by incubating in media...

07 May 2013 6,927 0 View

How to use the time information stored in the frames of a video in ImageJ instead of individually labeling the stacks?

I have a microscopy video (frames as stacks in a .tiff) which was acquired using a times series consisting of: 13 frames from time 0 to time 2:00, 1 frame taken every 10 s, 12 frames until 12:00...

12 April 2013 6,378 14 View

Amino acid concentrations in intracellular compartments in mammalian cells?

I am looking for some numbers of amino acid concentrations in intracellular compartments of mammalian cells (cytosol, endosome, lysosome,...). Extensive Google and Pubmed searches gave me a lot of...

17 March 2013 2,364 3 View