Maria Bikuña

7 Questions 6 Answers 0 Followers

Questions related from Maria Bikuña

Any recommendation for cell viability assays on porcine fat tissue?

Hi! I was wondering if anyone has performed live/dead assay (or MTT) on fat tissue. I not interested in obtaining the adipocytes I just want to process the whole tissue. Thank you!

09 December 2024 4,515 3 View

Any protocol for inmunofluorescence assay in electrospun meshes?

Hi! I am pursuing my research following the next protocol for inmuno assay in PCL electrospun meshes: - Fix the samples with 4 % PFA for 1h in RT - Clean 3X with DPBS for 3min each cleaning -...

12 May 2023 9,365 0 View

How can I visualize gelatin coated samples under SEM?

I would like to see an electrospun mesh under SEM after a coat with gelatin. My idea is to immerse de electrospun mesh in gelatin to perform the coating and then leave the sample in vacuum...

10 December 2021 4,310 1 View

How to improve oxygen solubility in cell media?

Hi everyone! I am working on a bioreactor measuring pressure differences. We have introduced a membrane with cells inside a chamber, which creates the pressure differences. The cell media is...

01 December 2021 810 4 View

Anyone was able to create a PCL/collagen disolution for the electropsinning process?

I have dissolved PCL 7.5% in chloroform:methanol (4:1) and collagen in aceti acid (Solubility: 0.1M for 1mg/mL). I mixed them in a PCL/collagen 4:1 concentraction. I was able to electrospin with...

29 January 2021 9,709 3 View

How can I fabricate nanofibers by electrospinning over a piece of glass instead of an aluminun foil?

I tried to electrospun PCL over a piece of glass with an aluminun foil just beneath. However, it suffers some repulsive forces that do not allow the propper deposition of the fibers ober the...

15 September 2020 4,088 3 View

Has anyone use PCL/gelatin for electrospinning process?

After the electrospinning process with PCL(20wt%) /gelatin (8wt%) at different concentrations: 50:50 and 75:25 I was unable to detach the mesh from the aluminun paper. I used chloroform:methanol...

02 September 2020 8,047 8 View