Manfredi Carta

6 Questions 2 Answers 0 Followers

Questions related from Manfredi Carta

Can someone recommend a flow cytometry protocol to sort astrocytes and microglia?

I would like to use flow cytometry sorting (Aria III) to sort astrocytes and microglia from adult human and adult mouse brain, with the aim of extracting RNA from sorted bulk pools. Can someone...

12 October 2020 3,742 1 View

Can you recommend some SARS-CoV2 monoclonal antibodies?

Hi all, I'm looking for some reliable monoclonal antibodies directed against the main SARS-CoV2 (Covid19) antigens Spike, RBD and Nucleocapsid (nucleoprotein) that work well in ELISA. I'd be...

12 May 2020 8,666 10 View

After centrifuging sorted nuclei, I can't see a pellet. Any ideas?

I am currently sorting neuronal (NeuN+) and non-neuronal (NeuN-) nuclei from human post-mortem brain tissue using a FACS Aria II. The FACS data look as expected: we gate for positivity for the...

29 November 2019 5,357 3 View

Is there a tool for finding a genomic locus with a particular GC content and length?

I am currently performing a sequencing experiment on a human gene of interest, where the gene is amplified using PCR. To verify performance of the PCR (number of artifacts etc), it would be good...

18 October 2019 9,882 1 View

How can I troubleshoot this 15kb long range PCR?

I am attempting to amplify a 15.4kb sequence from genomic DNA. The DNA template is high quality (fragment size >80kb) and I'm using the Generuler HR ladder. Gel is 0.4% agarose. I'm using the...

05 August 2019 8,830 2 View

How do you tag DNA barcodes to proteins?

I'm looking for simple ways to conjugate DNA tags to a variety of different proteins. Two possibilities I'm aware of is cloning a fusion protein consisting of the protein of interest bound to a...

01 February 2018 2,187 3 View