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I would like to use flow cytometry sorting (Aria III) to sort astrocytes and microglia from adult human and adult mouse brain, with the aim of extracting RNA from sorted bulk pools. Can someone...
12 October 2020 3,844 1 View
Hi all, I'm looking for some reliable monoclonal antibodies directed against the main SARS-CoV2 (Covid19) antigens Spike, RBD and Nucleocapsid (nucleoprotein) that work well in ELISA. I'd be...
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I am currently sorting neuronal (NeuN+) and non-neuronal (NeuN-) nuclei from human post-mortem brain tissue using a FACS Aria II. The FACS data look as expected: we gate for positivity for the...
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I am currently performing a sequencing experiment on a human gene of interest, where the gene is amplified using PCR. To verify performance of the PCR (number of artifacts etc), it would be good...
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I am attempting to amplify a 15.4kb sequence from genomic DNA. The DNA template is high quality (fragment size >80kb) and I'm using the Generuler HR ladder. Gel is 0.4% agarose. I'm using the...
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I am creating scFv phage display libraries using the VCSM13 helper phage (Agilent). It isn’t clear to me whether protein GIII can be cleaved by trypsin in this phage. To my knowledge its phenotype...
05 July 2019 3,292 2 View
I would like to fragment genomic DNA and cut out a gene of interest with restriction enzymes. To do so, I need a set of enzymes that cuts up- and downstream of my gene, but not within it. For...
05 April 2019 7,306 3 View
I'm looking for simple ways to conjugate DNA tags to a variety of different proteins. Two possibilities I'm aware of is cloning a fusion protein consisting of the protein of interest bound to a...
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