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Doing some RNA-FISH and I'd like to use Cy3-Tyramide and incorporated digoxigenein-d?TP (opinions? dUTP? dCTP? manufacturer? which nick translation kit?) to do some signal amplification. What's...
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I work in a big lab that is considering switching from IDT to Sigma to save on shipping costs. Has anybody used Sigma for ordering your oligos? What do you think about the quality of their...
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I'm trying to make a construct that I will use with TALENS to put a fluor at the AAVS1 locus. I have the AAVS1 left and right homolgy arms (HA's), but from a previous construct, there is an...
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