Mandy Herring

4 Questions 5 Answers 0 Followers

Questions related from Mandy Herring

What is your favorite Tyramide Signal Amplification Kit?

Doing some RNA-FISH and I'd like to use Cy3-Tyramide and incorporated digoxigenein-d?TP (opinions? dUTP? dCTP? manufacturer? which nick translation kit?) to do some signal amplification. What's...

08 July 2019 5,151 0 View

IDT vs Sigma?

I work in a big lab that is considering switching from IDT to Sigma to save on shipping costs. Has anybody used Sigma for ordering your oligos? What do you think about the quality of their...

10 March 2017 3,451 3 View

What is the insulator/blocker sequence in AAVS1 constructs used for?

I'm trying to make a construct that I will use with TALENS to put a fluor at the AAVS1 locus. I have the AAVS1 left and right homolgy arms (HA's), but from a previous construct, there is an...

26 October 2016 7,853 0 View

Anyone have experience with PSD-95 staining in human neurons differentiated with the Ngn2 protocol?

Hi all, I'm using DIV28 stem-cell derived Ngn2 neurons (DIV23 mouse neurons for comparison) and trying to stain for PSD-95. Cells are fixed in 4%PFA/4% sucrose at RT for 15 min and permeabilized...

07 April 2016 796 11 View