Hi people,
I performed some experiments in Ca1 pyramidal neurons to study sodium and potassium currents. I used classic I/V recordings (10 mV steps starting from -80mV with the cell clamped to -60). Of course i had sodium currents and also potassium, how i can analyse the trace to be sure to not over- (or under) estimate the current? Is sufficient to measure at the peak of the current?
Thanks in advance