11 February 2016 6 8K Report

Hi people,

I performed some experiments in Ca1 pyramidal neurons to study sodium and potassium currents. I used classic I/V recordings (10 mV steps starting from -80mV with the cell clamped to -60). Of course i had sodium currents and also potassium, how i can analyse the trace to be sure to not over- (or under) estimate the current? Is sufficient to measure at the peak of the current?

Thanks in advance

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