I am detecting my compound named indoxyl (which is highly unstable) in flurosence spectrophotometer using a standard indoxyl acetate.

I want to measure the same compound in HPLC -PDA .One reported literature said the compound has been identified by hydrolysing indoxyl acetate and releasing free indoxyl having maximum absorption at 240 nm. But free indoxyl is highly unstable . So how can i measure this compound?

More Lopa Pattanaik's questions See All
Similar questions and discussions