Liang Huang

6 Questions 11 Answers 0 Followers

Questions related from Liang Huang

Which is the best houskeeping gene for rtPCR in Enterobacter cloacae?

We are doing the rtPCR by using rpoD as a housekeeping gene, however, we found the rpoD was changing under the conditions of that with or without antibiotics. It is problematic. Do you have any...

04 March 2017 1,323 1 View

Is the the amino acid sequences similarity related to the affinity of proteins?

I was reading Errett C. Hobbsa 's paper, titled "Conserved small protein associates with the multidrug efflux pump AcrB and differentially affects antibiotic resistance" . In this paper the author...

31 May 2016 7,103 1 View

Is the Lac promoter inducible if the cloned gene has its own promoter ?

I knocked out my target gene, in order to confirm its function, I did the plasmid complement test. I cloned the gene with its full promoter into the MCS, however, it seems that the expression...

03 October 2015 2,521 6 View

How do I improve the efficiency of lambda red recombination?

I'm working on this, can anyone give any seggustion for improving the efficiency ? I did it for several times fruitlessly.

16 August 2015 315 9 View

Is it possible to select recombinant by using single resistant marker in lambda-red recombination system?

Here we have a problem with a multi-drug resistance bacteria (Enterobacter cloacae) in gene deletion by using pKD46. However, this strain is a clinical isolation, resistant for most antibiotics....

04 August 2015 3,922 6 View

How to extract the bacterial Lipid A for mass spectrum analysis?

I want to identify the modification of lipid A by mass spectrum analysis. However, the extraction is tricky.

01 January 1970 4,871 3 View