Liang Huang

4 Questions 9 Answers 0 Followers

Questions related from Liang Huang

Is the the amino acid sequences similarity related to the affinity of proteins?

I was reading Errett C. Hobbsa 's paper, titled "Conserved small protein associates with the multidrug efflux pump AcrB and differentially affects antibiotic resistance" . In this paper the author...

31 May 2016 7,097 1 View

Is the Lac promoter inducible if the cloned gene has its own promoter ?

I knocked out my target gene, in order to confirm its function, I did the plasmid complement test. I cloned the gene with its full promoter into the MCS, however, it seems that the expression...

03 October 2015 2,513 6 View

How do I improve the efficiency of lambda red recombination?

I'm working on this, can anyone give any seggustion for improving the efficiency ? I did it for several times fruitlessly.

16 August 2015 311 9 View

How to extract the bacterial Lipid A for mass spectrum analysis?

I want to identify the modification of lipid A by mass spectrum analysis. However, the extraction is tricky.

01 January 1970 4,864 3 View