Kyle Neal

4 Questions 4 Answers 0 Followers

Questions related from Kyle Neal

How to fix contamination in HPLC?

I am having contamination issues with my HPLC, specifically normal phase. We are running a gradient of 99.5% Hexane / 0.5 Ethyl Acetate down to 40% Hexane / 60% Ethyl acetate and the contamination...

14 December 2020 6,456 4 View

Why is only one of my solvent channels not pulling solvent (HPLC)?

I have an agilent 1260 infinity Quaternary Pump VL and am having an issue with my "D" channel not pulling an solvent through it. So far I have replaced the tubing from the solvent to the vacuum...

29 September 2020 2,274 3 View

How to get set up for analyzing TAGs on an LC MS/MS?

I am trying to get our new LC MS/MS set up to analyze triglycerides and am a little unsure about the proper solvents to use in order to get the best visualization. As it stands now I am going to...

15 September 2020 2,534 2 View

How do I get my proteins to show up on SDS PAGE?

I have been extracting protein from various seed meals but am having trouble visualizing them on my gel. I have tried two different types of gels, Tris-Acetate and Tricine, but so far neither have...

06 May 2020 8,651 4 View