Komal Kaushik

7 Questions 5 Answers 0 Followers

Questions related from Komal Kaushik

Can someone share a good protocol of sectioning and H& E for mice pancreas? I am facing problem in getting intact sections?

After sacrifice of animals, I am keeping pancreas in 4% paraformaldehyde for 1-2 weeks. Then for rehydration in 15% sucrose for 1 day before going for sectioning. I am using cryotome for making...

23 December 2016 9,084 7 View

How do I quantification and normalization a Ponceau stain?

Hi all , I have isolated proteins from Mesenchymal stem cells directly by using 3X loading buffer. I have done amido black staining for quantification. Further I have also done normalization by...

15 December 2015 1,595 1 View

Can anyone please help me to analyse microarray sample without replicates data using edgeR in R programming?

I have 3 time points mogene ST array without replicates. I would like to find differentially expressed genes using edgeR or any other method.

05 June 2015 2,884 1 View

Has anybody isolated protein from mouse mesenchymal stem cells? I am facing a problem with poor concentration of protein when using RIPA Lysis buffer?

Shall I change to any other harsh lysis buffer? Also I have run the gel on SDS PAGE I am getting one single band between 50- 75 Kda. Is it because of improper denaturation? Please help.

19 March 2015 4,388 5 View

What are the other methods to analyze microarray raw or processed files other than R programming?

Microarray from ncbi-GEO or array express analysis. what parameters do we need to confirm to use data from already existing array files on databases? how to analyze those microarrays whose...

05 March 2015 3,331 1 View

Can anybody tell me how to retrieve microarray data from Gene Expression Omnibus (GEO) repository at the NCBI?

microarray data for analysis

15 September 2014 4,427 4 View

Can I use recombinant human specific VEGF protein for mouse primary cells?

How can I check whether I can use that or not?

19 May 2014 5,495 4 View