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Questions related from Kimia Hosseini
"I have untreated cells as a control group and three concentrations of a drug. Each group consists of three replicate wells, and I conducted the same experiment three times. I want to compare the...
16 May 2023 7,736 1 View
Hello everyone. I have done drug exposure 2 days ago on my 96 well plates. I am not sure if I checked this particilar well before or not but today I saw this. It does not look like living! doesnt...
15 January 2023 6,904 7 View
Hello everyone! I am differentiating NSC to neurons for my new project that I need to expose with 3 different drugs! Everything looks perfect until I split the cells to 96 well plates and after...
28 December 2022 4,610 2 View
Hi! I have to dilute my drug (powder) in 100%DMSO. I need to have atleast 3 serial dilutions in 100% DMSO before the final dilution (0.1% dmso) in the media. the question is should I filter the...
19 December 2022 445 9 View
I have a bottle of drug powder that I need to dissolve in DMSO and add to my cells in the culture. the bottle was opened in non-sterlie condition. How can I know that there is no bacteria or fungi...
19 December 2022 9,931 1 View
I performed qpcr on my 3 biological replicates with untreated and treated. Delta ct values are very close together but when I try to calculate log2 delta delta the variance is huge between...
01 January 1970 8,718 0 View
