Stability of N-Benzyl substituted indazoles?

More Kelly Bugatti's questions See All

Qualitative Analysis Software Recommendations?

I am looking for expert recommendations on qualitative analysis software. I have been engaging in more mixed-method work recently and I need reliable qualitative analysis software. Any...

02 February 2021 7,984 7 View

How to tackle autofluorescence using the technique FISH in mouse frozen tissue?

Hi there, I am looking for some advice with FISH. This is my first time attempting this technique and I am not too sure where I could potentially be going astray. I have followed FISHTag RNA...

23 September 2020 3,849 1 View

What are the best emotional intelligence scales that contain subdimensions?

We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...

14 July 2020 7,122 2 View

How to normalize a Raman spectrum of graphene oxide to calculate ID / IG?

I am normalizing with respect to the G band, in this way I obtain as Raman intensity for this band a value of 1.00 and for the D band a value of 1.42, in this way I calculate ID / IG = 1.42, I...

21 December 2019 6,923 3 View

CAMKII-alpha triple immunofluorescence help in Primate?

I'm having a heck of time! I'm trying to perform triple immunofluorescence for CAMKII (alpha; mouse; milipore 05-532; 1:5K; visualized with AlexaFluor 647), Fluorescein (Invitrogen, A11095, goat,...

29 October 2019 7,063 2 View

Phosphorylated and non-phosphorylated protein X in the same western via IR fluorescence secondary?

The size of protein X (Phosphorylated and non-phosphorylated) will be pretty much the same on a western. Let's say after primary antibodies incubation of the two separate proteins (Phosphorylated...

16 September 2019 5,583 5 View

Oil Red O staining protocol - best mount to use?

Hi, I am having trouble with my Oil Red O stain for my rat MSCs. What is the best aqueous mounting medium to use? I followed the protocol from IHCWorld...

03 April 2019 10,012 3 View

Statistical Method for Treatment Outcome Research?

Hello all, I am completing a paper on the change in impairment over the course of treatment. I am not only interested if seeing that there is change overall, but where the biggest change occurs...

24 February 2019 3,709 4 View

Quantification of osteoblast staining?

Hi, I am planning on seeding rat MSCs onto 6 well plates with coverslips attached on the bottom. I then want to use stains such as Alizarin Red and Von Kossa to see the level of osteogenic...

02 February 2019 7,584 1 View

How do I get started with a Research Action Project for my Capstone course?

I am totally confused after beginning this course a week ago. However, I want to fully understand so that I can make the best grade possible. I have considered topics dealing with Bullying,...

13 January 2019 5,876 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Frank T. Edelmann

Dear Kelly Bugatti , as you can see from the attached article, N-benzyl substituted indazoles can be debenzylated in the presence of oxygen under basic conditions.

Abdelkader BOUAZIZ

Dear all, besides the reason given by Prof. Frank T. Edelmann, the following MSc thesis discuss in the first chapter the involvement of toutomerism and idomerism to its intrinsic unstability because of geometrical contraints. Details are presented in the references mentioned. My Regards

Kelly Bugatti

Thanks for your precious answers!