Karen Butina

11 Questions 23 Answers 0 Followers

Questions related from Karen Butina

Non-biological recognition elements for bacterial sensors?

Hi, I am interested in non-biological recognition elements for bacterial sensors. I am aware of molecularly imprinted polymers but I do not seem to find many other alternatives. I know that many...

19 September 2020 6,034 3 View

Way to oxidize flavins and related molecules?

I would like to oxidize flavins and if possible also quinones in bacterial supernatant. Is there an enzyme one could use for that? Or any other "oxidant"? I use M9 minimal medium. Thank you so...

26 November 2017 2,452 3 View

Can one fix bacteria with isopropyl alcohol?

I would like to fix S.aureus on plastic surfaces and I cannot use EtOH or acetone since they dissolve my plastics and I would just like to try another method then formaldehyde. Than you so...

10 October 2017 8,200 3 View

Bulk electrical conductivity calculated from impedance modulus?

I have come across a publication (Jafarzadeh et al., 2014) where they have calculated bulk electrical conductivity of polymer film coated on metal from impedance modulus using the...

12 September 2017 5,788 3 View

Is there a transposon library of S.aureus 8325-4 one can obtain?

I am looking for transposon library in S.aureus 8325-4 strain.

11 July 2017 4,281 5 View

Sequential spin coating of the same material to increase film thickness?

I am working with PEDOT:PSS thin films that I routinely produce by spin coating on play microscope slides. For various measurements I would need to have films of different...

05 July 2017 5,390 8 View

What is a good method for cleaning PET before spin coating?

I am trying to spin coat PEDOT:PSS on PET but I cannot obtain homogeneous surfaces. So far, I have tried to clean PET with 15 min plasma treatment only but I guess I would need to introduce a...

31 March 2017 5,700 3 View

Liquid junction potential -- no current flow?

I have a very simple question - let´s assume we have two electrodes immersed in different solutions, connected with a bridge made of a porous, non-conductive polymer, not saturated in salts. is it...

05 January 2016 6,366 4 View

Does Iodoactamide not work on LMW thiols?

hej, I am having troubles with something that is supposed to be quite trivial, so I am most likely doing something stupid and would be very happ if somebody tells me what is it : ) So, I dissolve...

31 October 2015 9,816 3 View

Can anyone suggest other tags for purifying proteins under denaturing conditions other than Histidine-Tagged?

We would like to find an option to purify proteins under denaturing conditions (= 6M guanidinium hydrochloride) that is not his column (or similar, cobalt etc). Any ideas? Thanks a lot!

25 February 2015 939 10 View

Why am I unable to blot a recombinant protein from mouse liver ?

We have used hydrodynamic injection (http://www.mjhid.org/article/view/418/620) to deliver DNA to liver where we have shown with microscopy and IHC that the protein is present. We have also used...

07 February 2015 1,952 11 View