Kamal Adhikari

5 Questions 2 Answers 0 Followers

Questions related from Kamal Adhikari

Is there any Cytotoxicity assay that has been reported for the novel polyketides product generated by engineering plant Polyketide Synthase Type III?

I am engineering polyketide synthase III to generate unnatural (novel) Polyketides products. I would like to test its cytotoxicity. Is there any PKS-III novel product use for cytotoxicity assay...

26 December 2015 9,049 2 View

How does active site cavity amino acid residues influence the pattern of cyclization and intermediate product in polyketides synthase?

i am just the beginner in Polyketides Synthase III (PKSsIII). I am curious how does the amino acid residue influence the intermediate chain elongation, cyclization pattern and alter the...

17 July 2015 7,781 1 View

Can we use MTT-assay for high-throughput screening (HTS) for determining the cytotoxicity of drugs?

I am curious whether MTT can be use for HTS or not? i need to determine the cytotoxicity of 50 different small compounds against various panel of cancer cell line (7 cancer cell line). Is it...

02 July 2015 5,098 3 View

Site-directed mutagenesis.

I am doing site-directed mutagenesis for DNMT1 protein. I used PCR master mix as my DNA polymerase. After PCR, I used DPN1 enzyme to digest my paternal plasmid (dpnl: a restriction enzyme which...

19 November 2012 5,021 2 View

Anyone suggest an efficient method for the synthesis of Fluoromalonate and Aminomalonate?

I used diethyl 2-fluromalonate and diethyl 2-aminomalonate as a starting materials. Both of this were saponified with methanolic sodium hydroxide (2 M, 3.5 mL) in dichloromethane and methanol (9:1...

01 January 1970 3,238 1 View