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Questions related from Kamal Adhikari
I am trying to do antioxidant assay for resveratrol derivatives , is there any specific antioxidant assay kit for Resveratrol? Thanking you on advance.
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I am engineering polyketide synthase III to generate unnatural (novel) Polyketides products. I would like to test its cytotoxicity. Is there any PKS-III novel product use for cytotoxicity assay...
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i am just the beginner in Polyketides Synthase III (PKSsIII). I am curious how does the amino acid residue influence the intermediate chain elongation, cyclization pattern and alter the...
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I am curious whether MTT can be use for HTS or not? i need to determine the cytotoxicity of 50 different small compounds against various panel of cancer cell line (7 cancer cell line). Is it...
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I am using Arabidopsis thaliana gene (4-coumaroyl-coa ligase 3) At4CL3 to express through E,coli strain BL21 (De3) strain. I used Pet28a expression vector to express the protein with 6xhis tag. I...
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I am doing site-directed mutagenesis for DNMT1 protein. I used PCR master mix as my DNA polymerase. After PCR, I used DPN1 enzyme to digest my paternal plasmid (dpnl: a restriction enzyme which...
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I used diethyl 2-fluromalonate and diethyl 2-aminomalonate as a starting materials. Both of this were saponified with methanolic sodium hydroxide (2 M, 3.5 mL) in dichloromethane and methanol (9:1...
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