8 Questions 13 Answers 0 Followers
Questions related from Jyoti Sharma
The HGP project utilized white blood cells from female and sperm cells from male candidates to map the human DNA. Since these cells are differentiated, the ratio of heterochromatin to euchromatin...
10 October 2019 5,577 1 View
Dear research community I was reading a paper where for making a conditional knockout in a chicken cell line, researchers have used cDNA instead of a gene for that particular protein. I wanted to...
08 August 2019 8,094 5 View
I am using 4 gel units of MNase enzyme for 2ug of polynucleosomes in expectation to get mononucleosomes. I kept this reaction at 37degC for 15 minutes. This reaction worked two times, confirmed by...
07 July 2019 3,898 4 View
I am trying to make a clone using E coli as host and use that clone later to transform budding yeast. But, I am not able to get clone somehow so I was wondering to perform lithium acetate...
12 December 2018 3,028 16 View
I am planning to introduce a point mutation in Saccharomyces cerevisiae, strain background CEN.PK. So, I was curious in asking whether instead of adding mutation using primers, joining mutated...
10 October 2018 1,569 0 View
Currently, I am working on yeast strain CEN PK and I want to carry out TUNEL Assay to detect amount of damage upon treatment with some damaging agent. We have a kit in the name of TUNEL Assay Kit...
05 May 2018 2,983 3 View
Out of curiosity, I got this question whether knocking out (deletion) of a gene on one side and knocking down (RNAi) of the same gene on the other side will affect the cell in a similar manner or...
04 April 2018 7,894 9 View
I have been working on yeast cells to analyse the effect of DNA damaging agents on mitochondrial structure. I have imaged my culture treated with MMS for a period of 6 hours and while observing...
03 March 2018 6,676 4 View
