Joshua Paul

4 Questions 5 Answers 0 Followers

Questions related from Joshua Paul

How do we standardize the volume of broth culture and hours of incubation to achieve a desired concentration of my plasmid?

After transformation, we plated it for overnight and the next day we inoculated a single colony into 5 ml LB broth culture and got an OD of 1.099 after 8 Hrs. Now, how much inoculum should be used...

27 November 2021 8,253 3 View

What is the mechanism of endotoxins being removed from the lysate in endofree plasmid isolation protocols?

In most of the commercial kits, they have a proprietary formulation which is not disclosed.

11 November 2021 280 4 View

Has anyone optimized PEI-based transfection of HEK-293 cells? What is the best PEI available for large-scale virus production?

Polyethylenimine (PEI) is a polymer used for transfection

08 November 2021 2,391 3 View

Are there any Research Management System (RMS) available? How common it is used in research labs?

RMS is a software application where we document, monitor, track and administer all information in a research lab including personnel, projects, funding, reagents and equipment. So, this makes...

01 January 1970 8,938 4 View