Joshua Paul

3 Questions 4 Answers 0 Followers

Questions related from Joshua Paul

How do we standardize the volume of broth culture and hours of incubation to achieve a desired concentration of my plasmid?

After transformation, we plated it for overnight and the next day we inoculated a single colony into 5 ml LB broth culture and got an OD of 1.099 after 8 Hrs. Now, how much inoculum should be used...

27 November 2021 8,242 3 View

What is the mechanism of endotoxins being removed from the lysate in endofree plasmid isolation protocols?

In most of the commercial kits, they have a proprietary formulation which is not disclosed.

11 November 2021 269 4 View

Has anyone optimized PEI-based transfection of HEK-293 cells? What is the best PEI available for large-scale virus production?

Polyethylenimine (PEI) is a polymer used for transfection

08 November 2021 2,376 3 View