12 Questions 6 Answers 0 Followers
Questions related from Joseph Compton Madden
I am performing conjugations where I am adding oligonucleotides to proteins. For my confirmation steps, I am doing SDS-PAGE and staining the gel using Pierce Silver Stain to observe shifts in the...
01 January 2020 3,878 3 View
We are analzying changes in miRNA expression as a result of virus infection using Nanostring. While I have a lot of interesting hits in my data, I do not know the best way to determine the...
10 October 2019 8,533 3 View
We want to make some custom antibodies against viral nonstructural proteins. We wish to only provide the primary amino acid sequences. We did so in the past, but the company we worked with does...
01 January 2015 10,075 6 View
Specifically, does ethidium bromide bind with equal efficiency to single stranded DNA as to double stranded? Is there a good resource for learning about the chemistry behind EB? THANKS!
10 October 2014 2,596 4 View
How can I purify a PCR product that is smaller than 70 bp? I typically use a Qiagen gel purification prep to purify PCR; however, the manual states that fragments of that size are removed rather...
02 February 2014 7,112 13 View
Using immunofluorescence, I hope to study the interaction of a mouse protein, which contains a transmembrane domain, and viral replication complexes. Unfortunately, no effective antibodies for the...
01 January 2014 2,068 15 View
I have glycerol stocks of transformed competent cells containing p3xFLAG-CMV-10 with ampicillin resistance and my gene of interest. The gene is a mouse oligodenylate synthetase protein with a...
11 November 2013 1,718 3 View
Whenever I use the Bio-Rad protein assay to measure my protein level in RIPA buffer, I use the RIPA buffer as a blank. However, it gives a color change as well! I have attempted several fixes, but...
10 October 2013 5,708 26 View
I need to isolate these fragments from a PCR reaction and then sequence them to see if they contain a conserved sequence. I have predicted product sizes that I am looking for, and while being able...
08 August 2013 8,044 21 View
I have seen the answer from the manufacturer, but I was curious if anybody has had any luck using them interchangeably
06 June 2013 2,972 3 View
I was doing some cloning, and everything was going swimmingly. I only used a single restriction enzyme for the insertion (I know, I know, so sue me). Anyway, the gene was inserted in the incorrect...
06 June 2013 9,157 9 View
Accidently used Elution buffer instead of wash when purifying PCR products and plasmids in Qiaquik columns. Currently, I was wondering if anybody knew if it was possible to recover the DNA, or do...
06 June 2013 4,245 6 View
