Joe Joe

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Questions related from Joe Joe

Protein expression after gapmer treatment?

Hi all, I have been trying to show changes in protein expression of my target after gapmer treatment. I used multiple gapmer ASOs and sirnas for my target. while all can reduce mRNA expression by...

13 December 2024 2,808 0 View

Co-transfection plasmid and sirna problem?

Hi folks, I have been trying to co-transfect plasmid and siRNA in HeLa by using reverse transfection with Lipofectamine 2000 (L2K) in 96 well plate for a reporter (psiCHECK-2) based screen. I...

26 September 2024 3,323 2 View

How to prepare viscous cosmetic product for in vitro assay?

Dear all, I would like to know how you prepare viscous cosmetic products such as serums, creams etc. for in vitro assays such as cell viability, ELISA etc.? I want to know how you adjust the...

17 December 2022 1,148 7 View