Jessica Little

5 Questions 3 Answers 0 Followers

Questions related from Jessica Little

Lots of growth on conjugation negative control compared to actual conjugation plate?

Hi all, I am trying to conjugate a plasmid from E.coli ET12567 into anAmycolatopsis strain. The plasmid is integrative so should integrate into the genome of Amycolatopsis. I have a negative and...

26 July 2022 3,691 2 View

Can you add 2 of the same gene to an integrative vector?

Hi, Is it possible to add 2 of the same genes under the same promoter to an integrative vector? My goal is gene upregulation so I am adding a strong promoter to the gene and want to add 2 copies...

09 March 2022 6,333 3 View

How to view promoter sequences in Snapgene?

Hi, I have just started using snapgene and have downloaded a plasmid sequence off addgene to have a play with. Addgene says a gene is under control of a KasOp promoter, but when I insert the...

11 February 2022 1,315 1 View

Constitutive vs Inducible promoter engineering for overexpression of secondary metabolites (antibiotics)?

Hi everyone, I am hoping to overproduce an antibiotic by swapping promoters. I am just wondering what the advantages/ disadvantages using inducible/constitutive promoters for the overexpression...

03 August 2021 4,251 2 View

Tricine SDS-PAGE advice?

Hi everyone, I’m wondering if you could help I’ve ran a Tricine SDS-PAGE to help visualise a 2kDa glycopeptide produced by bacteria and there are several issues. The gel is a MINI-PROTEAN...

16 February 2021 3,586 3 View