Jessica Little

9 Questions 6 Answers 0 Followers

Questions related from Jessica Little

Decreasing Glycopeptide Antibiotic Concentration over time?

Hi, I am just wondering if anyone has experienced this before? I have been doing a time-course of glycopeptide antibiotic yield from liquid culture of actinomycetes to determine the...

23 February 2023 8,171 0 View

Are NRPSs in a gene cluster used for the production of more than one molecule in the same organism?

Hi, I am just wondering whether NRPSs in a gene cluster are specifically used solely for the final product of that gene cluster or whether the NRPS can be utilised to produce a compound from...

16 February 2023 1,794 0 View

Lots of growth on conjugation negative control compared to actual conjugation plate?

Hi all, I am trying to conjugate a plasmid from E.coli ET12567 into anAmycolatopsis strain. The plasmid is integrative so should integrate into the genome of Amycolatopsis. I have a negative and...

26 July 2022 3,699 2 View

Can you add 2 of the same gene to an integrative vector?

Hi, Is it possible to add 2 of the same genes under the same promoter to an integrative vector? My goal is gene upregulation so I am adding a strong promoter to the gene and want to add 2 copies...

09 March 2022 6,348 3 View

How to view promoter sequences in Snapgene?

Hi, I have just started using snapgene and have downloaded a plasmid sequence off addgene to have a play with. Addgene says a gene is under control of a KasOp promoter, but when I insert the...

11 February 2022 1,327 1 View

Why am I getting large variation in antibiotic production levels across triplicate co-cultures?

Hi there, I am currently co-culturing B. subtilis (B) and an Acinomycetes (A) to induce expression of a glycopeptide antibiotic by the actinomycetes. The actinomycetes strain is quite slow...

23 November 2021 9,973 6 View

Constitutive vs Inducible promoter engineering for overexpression of secondary metabolites (antibiotics)?

Hi everyone, I am hoping to overproduce an antibiotic by swapping promoters. I am just wondering what the advantages/ disadvantages using inducible/constitutive promoters for the overexpression...

03 August 2021 4,262 2 View

How to induce glycopeptide antibiotic production?

Hi, I have a bacteria that is known to produce a glycopepide antibiotic, but I can't seem to get it to produce it or i am unable to detect it? Does anyone have expertise in this field who could...

07 March 2021 4,649 1 View

Tricine SDS-PAGE advice?

Hi everyone, I’m wondering if you could help I’ve ran a Tricine SDS-PAGE to help visualise a 2kDa glycopeptide produced by bacteria and there are several issues. The gel is a MINI-PROTEAN...

16 February 2021 3,604 3 View