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Questions related from Jakob Zimmermann
We're looking for a working PCR protocol to genotype Ahr-deficient mice (Schmidt et al., PNAS, 1996). We're facing a few issues with our current protocol and would be grateful for a protocol that...
23 July 2019 6,006 0 View
We're looking for a working PCR protocol to genotype Il17a-Cre mice (Hirota, Nat Immunol, 2011). We're facing a few issues with the protocol recommended on the Jackson website and would be...
14 July 2019 8,339 1 View
To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...
23 October 2017 6,430 3 View
Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...
13 September 2017 2,572 2 View
Has anybody tried isolating RNA from formaldehyde-fixed bacteria, ideally not paraffin-embedded but just in solution? Is the integrity preserved, i.e. clear peaks for the ribosomal RNAs,...
06 September 2016 1,930 2 View
We are struggling with the staining of murine TLR2 in PFA-fixed mouse cryosections. There are antibodies that work well for flow but up to now they didn't stain anything at all in PFA-fixed...
27 May 2014 6,535 1 View
