10 Questions 26 Answers 0 Followers
Questions related from Jakob Zimmermann
I have encoded log2FC by size in STRING networks built with cytoscape (3.7.2). When exporting them, the log2FC key is not comparable across different networks as cytoscape exports either the...
10 April 2020 5,171 0 View
We're looking for a working PCR protocol to genotype Ahr-deficient mice (Schmidt et al., PNAS, 1996). We're facing a few issues with our current protocol and would be grateful for a protocol that...
23 July 2019 6,066 0 View
We're looking for a working PCR protocol to genotype Il17a-Cre mice (Hirota, Nat Immunol, 2011). We're facing a few issues with the protocol recommended on the Jackson website and would be...
14 July 2019 8,393 1 View
Does anyone know a reference for the concentration of dextran sulfate sodium that is reached in the colonic lumen when given to mice in the drinking water as a 1/2/3 % solution?
09 April 2019 2,572 0 View
Is there any study out there where the genetic risk for IBD has been addressed in the context of a certain microbiota composition, say enterotype, or according to presence/absence of indicator...
10 November 2017 1,251 3 View
To enumerate the total amount of bacteria per blood volume, I would like to selectively remove host cells from mouse blood while retaining bacteria. Size filtration is unlikely to work as filters...
23 October 2017 6,561 3 View
Is there any study around on how well bacterial transcriptomes are preserved when they are taken out of the "in vivo" situation and kept on ice for varying amounts of time? In other words, how...
13 September 2017 2,686 2 View
Has anybody tried isolating RNA from formaldehyde-fixed bacteria, ideally not paraffin-embedded but just in solution? Is the integrity preserved, i.e. clear peaks for the ribosomal RNAs,...
06 September 2016 2,002 2 View
We are struggling with the staining of murine TLR2 in PFA-fixed mouse cryosections. There are antibodies that work well for flow but up to now they didn't stain anything at all in PFA-fixed...
27 May 2014 6,604 1 View
I wonder whether it is known by now where the CD4-Cre transgene established by Christopher B. Wilson integrated into the DNA and whether therefore a conventional PCR would be possible to...
18 March 2014 5,777 3 View
