Itseng Saudu

4 Questions 3 Answers 0 Followers

Questions related from Itseng Saudu

Is it correct to calculate percentage using log values than actual values?

Im calculating the recovery rate of cultures before and after freeze drying by dividing the after freeze drying counts with before freezing counts and multiplying with 100. Im working in excel and...

08 October 2019 2,701 3 View

What is the accurate way or formula for estimating bacterial counts in the original sample by serially diluting it and plating sevaral dilutions?

I want to know how to estimate bacterial counts in the original sample from its serial dilutions when (a) two plates from successive dilutions are counted, (b) one plate from two successive...

26 September 2019 2,856 3 View

How can one maximise the vapor pressure of water in a frozen eutectic mixture during freeze drying?

I freeze dry bacterial suspensions using an old shelf freeze dryer. I freeze my suspensions in a -80oC freezer overnight before transferring samples on to the shelves of a drying chamber which is...

21 August 2019 3,872 1 View

How does one determine the ideal cryoprotectants for a particular bacterial culture.? 10% skim milk and 20% glycerol have not been effective so far?

My cultures are grown on tryptone soya broth (TSB) and nutrient agar slants. Those on broth are centrifuged and pellet suspended in the same growth broth with equal volume of 20% glycerol before...

27 July 2018 5,756 3 View