Imtiaj Uddin

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Questions related from Imtiaj Uddin

Insights on common challenges in protein purification and how you address them?

22 September 2025 1,066 3 View

Confusion in Ni-Affinity Chromatography (Post-His-Tag Removal) Step – Where is my protein eluting?

Question: I am facing issues in the Ni-affinity chromatography step after His-tag removal (Post-His-tag removal purification). I loaded about 15 mL of my protein sample onto the column. I...

13 September 2025 8,888 4 View

How will I wash His Trap HP 5 ml column before and after using for protein purification?

How will I wash His Trap HP 5 ml column before and after using it for protein purification?

11 September 2025 4,028 2 View

Which flow-through fractions should be collected after His-tag removal using Ni-affinity chromatography?

I am performing protein purification on an ÄKTA Prime Plus system. After His-tag affinity purification, I cleave the His-tag from my protein with thrombin. In the post-His-tag removal step, I...

06 September 2025 5,315 3 View

AKTAprime plus guidance: handling medium-volume (20 mL) protein samples?

I am a beginner in protein purification and currently learning to use the AKTAprime plus system for His-tagged protein purification. I would like some guidance on practical...

29 August 2025 5,820 2 View