I want to give salt treatment to my plants. I have grown them in soil in greenhouse (weight of soil in pot is 1.2 kg). I want to give 200mM NaCl stress treatment to these plant and then at different time interval (Control, 6h, 12h,18, 24h) I am going to isolate RNA. So how much saline water I should add to this 1.2 kg of soil to give 200mM stress.
In hydroponics we can easily calculate the salt treatment. as my plant is upland plant (susceptible to waterlogging) it doesn't grow well in hydroponics.
How old plant is good for salt stress? actually plant i am working is millet and in 10 DAS it produces 4-5 leaves.
Please guide me. Any suggestion or guidance will be ok.
Thanks and regards
Harshraj